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axio observer microscope  (Carl Zeiss)
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Carl Zeiss axio observer microscope
Axio Observer Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted fluorescence microscope  (Carl Zeiss)
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Carl Zeiss inverted fluorescence microscope
Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio observer 7 microscope  (Carl Zeiss)
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Carl Zeiss axio observer 7 microscope
Axio Observer 7 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colibri 7 fluorescent led illumination  (Hamamatsu)
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colibri 7  (Carl Zeiss)
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Carl Zeiss colibri 7
Colibri 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solid state light source colibri 7  (Carl Zeiss)
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Carl Zeiss solid state light source colibri 7
Solid State Light Source Colibri 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orca-flash4.0 camera hamamatsu #c13440-20cu  (Hamamatsu)
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solid state colibri 7 led illuminator  (Nikon)
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Nikon solid state colibri 7 led illuminator
Solid State Colibri 7 Led Illuminator, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tissuefaxs plus system  (TissueGnostics)
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fluorescence camera  (Carl Zeiss)
96
Carl Zeiss fluorescence camera
AIS development in different neuronal cell systems shows classic AIS structure in iN and primary mouse neurons with marked AnkG and TRIM46 enrichment, in contrast to SHN with AnkG deficiency and moderate TRIM46 enrichment in the proximal axon. (a1–3) Representative images of iN at day 17 post-differentiation (d17) (a1), differentiated SHN (a2) and primary mouse neurons (mPN), 9 days in vitro (DIV9) (a3), immuno-labeled with AnkG (blue) and TRIM46 (green) and MAP2 (red) antibody. Note clear AIS staining in the proximal axon of iN and mPN, but absent AnkG staining and moderate TRIM46 staining in SHN. (b1–3) <t>Fluorescence</t> profiles of AnkG and TRIM46 staining in the proximal axon. Note that absolute values were normalized to a maximum of iN plot (b1, b2), or the maximum of primary neurons plot (b3). Data from B2 and B3 were obtained from Michael Bell-Simons for comparison . The AIS profile appears typical for both AIS proteins in iN and mPN. In contrast, relatively low TRIM46 levels and negligible antibody labeling for AnkG in SHN. (c) AIS enrichment factor for AnkG (orange) and TRIM46 (green) in the three cell types. TRIM46 is much less locally enriched (∼1.6 fold) in SHN than in the other neuronal cell models. (d) qPCR of AnkG and TRIM46 mRNA levels reveal no AnkG expression, but increasing TRIM46 expression during differentiation in SH-SY5Y cells. Statistics: (b1–3) Analysis was done from 3 to 4 experimental replicates with 15–20 neurons each per cell type with a two-way analysis of variance with post hoc Sidak’s multiple comparisons. The alpha level is 0.05, and variance is between groups. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001. Standard deviation is indicated by area fill around line (b) or error bars (c). (d) Analysis was done from two experimental replicates per cell type with 3 technical replicates each.
Fluorescence Camera, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio imager a2 microscope  (Carl Zeiss)
96
Carl Zeiss axio imager a2 microscope
AIS development in different neuronal cell systems shows classic AIS structure in iN and primary mouse neurons with marked AnkG and TRIM46 enrichment, in contrast to SHN with AnkG deficiency and moderate TRIM46 enrichment in the proximal axon. (a1–3) Representative images of iN at day 17 post-differentiation (d17) (a1), differentiated SHN (a2) and primary mouse neurons (mPN), 9 days in vitro (DIV9) (a3), immuno-labeled with AnkG (blue) and TRIM46 (green) and MAP2 (red) antibody. Note clear AIS staining in the proximal axon of iN and mPN, but absent AnkG staining and moderate TRIM46 staining in SHN. (b1–3) <t>Fluorescence</t> profiles of AnkG and TRIM46 staining in the proximal axon. Note that absolute values were normalized to a maximum of iN plot (b1, b2), or the maximum of primary neurons plot (b3). Data from B2 and B3 were obtained from Michael Bell-Simons for comparison . The AIS profile appears typical for both AIS proteins in iN and mPN. In contrast, relatively low TRIM46 levels and negligible antibody labeling for AnkG in SHN. (c) AIS enrichment factor for AnkG (orange) and TRIM46 (green) in the three cell types. TRIM46 is much less locally enriched (∼1.6 fold) in SHN than in the other neuronal cell models. (d) qPCR of AnkG and TRIM46 mRNA levels reveal no AnkG expression, but increasing TRIM46 expression during differentiation in SH-SY5Y cells. Statistics: (b1–3) Analysis was done from 3 to 4 experimental replicates with 15–20 neurons each per cell type with a two-way analysis of variance with post hoc Sidak’s multiple comparisons. The alpha level is 0.05, and variance is between groups. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001. Standard deviation is indicated by area fill around line (b) or error bars (c). (d) Analysis was done from two experimental replicates per cell type with 3 technical replicates each.
Axio Imager A2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescent+microscope+axio+observer+colibri+7/Axio+Imager%2EA2+microscope+stand+with+LED/pmc08619261-98-6-19
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stage heater  (Tokai Hit Co Ltd)
86
Tokai Hit Co Ltd stage heater
AIS development in different neuronal cell systems shows classic AIS structure in iN and primary mouse neurons with marked AnkG and TRIM46 enrichment, in contrast to SHN with AnkG deficiency and moderate TRIM46 enrichment in the proximal axon. (a1–3) Representative images of iN at day 17 post-differentiation (d17) (a1), differentiated SHN (a2) and primary mouse neurons (mPN), 9 days in vitro (DIV9) (a3), immuno-labeled with AnkG (blue) and TRIM46 (green) and MAP2 (red) antibody. Note clear AIS staining in the proximal axon of iN and mPN, but absent AnkG staining and moderate TRIM46 staining in SHN. (b1–3) <t>Fluorescence</t> profiles of AnkG and TRIM46 staining in the proximal axon. Note that absolute values were normalized to a maximum of iN plot (b1, b2), or the maximum of primary neurons plot (b3). Data from B2 and B3 were obtained from Michael Bell-Simons for comparison . The AIS profile appears typical for both AIS proteins in iN and mPN. In contrast, relatively low TRIM46 levels and negligible antibody labeling for AnkG in SHN. (c) AIS enrichment factor for AnkG (orange) and TRIM46 (green) in the three cell types. TRIM46 is much less locally enriched (∼1.6 fold) in SHN than in the other neuronal cell models. (d) qPCR of AnkG and TRIM46 mRNA levels reveal no AnkG expression, but increasing TRIM46 expression during differentiation in SH-SY5Y cells. Statistics: (b1–3) Analysis was done from 3 to 4 experimental replicates with 15–20 neurons each per cell type with a two-way analysis of variance with post hoc Sidak’s multiple comparisons. The alpha level is 0.05, and variance is between groups. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001. Standard deviation is indicated by area fill around line (b) or error bars (c). (d) Analysis was done from two experimental replicates per cell type with 3 technical replicates each.
Stage Heater, supplied by Tokai Hit Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


AIS development in different neuronal cell systems shows classic AIS structure in iN and primary mouse neurons with marked AnkG and TRIM46 enrichment, in contrast to SHN with AnkG deficiency and moderate TRIM46 enrichment in the proximal axon. (a1–3) Representative images of iN at day 17 post-differentiation (d17) (a1), differentiated SHN (a2) and primary mouse neurons (mPN), 9 days in vitro (DIV9) (a3), immuno-labeled with AnkG (blue) and TRIM46 (green) and MAP2 (red) antibody. Note clear AIS staining in the proximal axon of iN and mPN, but absent AnkG staining and moderate TRIM46 staining in SHN. (b1–3) Fluorescence profiles of AnkG and TRIM46 staining in the proximal axon. Note that absolute values were normalized to a maximum of iN plot (b1, b2), or the maximum of primary neurons plot (b3). Data from B2 and B3 were obtained from Michael Bell-Simons for comparison . The AIS profile appears typical for both AIS proteins in iN and mPN. In contrast, relatively low TRIM46 levels and negligible antibody labeling for AnkG in SHN. (c) AIS enrichment factor for AnkG (orange) and TRIM46 (green) in the three cell types. TRIM46 is much less locally enriched (∼1.6 fold) in SHN than in the other neuronal cell models. (d) qPCR of AnkG and TRIM46 mRNA levels reveal no AnkG expression, but increasing TRIM46 expression during differentiation in SH-SY5Y cells. Statistics: (b1–3) Analysis was done from 3 to 4 experimental replicates with 15–20 neurons each per cell type with a two-way analysis of variance with post hoc Sidak’s multiple comparisons. The alpha level is 0.05, and variance is between groups. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001. Standard deviation is indicated by area fill around line (b) or error bars (c). (d) Analysis was done from two experimental replicates per cell type with 3 technical replicates each.

Journal: Open Life Sciences

Article Title: Axodendritic targeting of TAU and MAP2 and microtubule polarization in iPSC-derived versus SH-SY5Y-derived human neurons

doi: 10.1515/biol-2022-1010

Figure Lengend Snippet: AIS development in different neuronal cell systems shows classic AIS structure in iN and primary mouse neurons with marked AnkG and TRIM46 enrichment, in contrast to SHN with AnkG deficiency and moderate TRIM46 enrichment in the proximal axon. (a1–3) Representative images of iN at day 17 post-differentiation (d17) (a1), differentiated SHN (a2) and primary mouse neurons (mPN), 9 days in vitro (DIV9) (a3), immuno-labeled with AnkG (blue) and TRIM46 (green) and MAP2 (red) antibody. Note clear AIS staining in the proximal axon of iN and mPN, but absent AnkG staining and moderate TRIM46 staining in SHN. (b1–3) Fluorescence profiles of AnkG and TRIM46 staining in the proximal axon. Note that absolute values were normalized to a maximum of iN plot (b1, b2), or the maximum of primary neurons plot (b3). Data from B2 and B3 were obtained from Michael Bell-Simons for comparison . The AIS profile appears typical for both AIS proteins in iN and mPN. In contrast, relatively low TRIM46 levels and negligible antibody labeling for AnkG in SHN. (c) AIS enrichment factor for AnkG (orange) and TRIM46 (green) in the three cell types. TRIM46 is much less locally enriched (∼1.6 fold) in SHN than in the other neuronal cell models. (d) qPCR of AnkG and TRIM46 mRNA levels reveal no AnkG expression, but increasing TRIM46 expression during differentiation in SH-SY5Y cells. Statistics: (b1–3) Analysis was done from 3 to 4 experimental replicates with 15–20 neurons each per cell type with a two-way analysis of variance with post hoc Sidak’s multiple comparisons. The alpha level is 0.05, and variance is between groups. Significance levels: * p < 0.05, ** p < 0.01, *** p < 0.001. Standard deviation is indicated by area fill around line (b) or error bars (c). (d) Analysis was done from two experimental replicates per cell type with 3 technical replicates each.

Article Snippet: Microscopy images were taken with a widefield fluorescence microscope (Zeiss), equipped with an LED lamp (Colibri 7, Zeiss) and a fluorescence camera (Axiocam 503 mono, Zeiss).

Techniques: In Vitro, Labeling, Staining, Fluorescence, Comparison, Antibody Labeling, Expressing, Standard Deviation